This award helped catalyze a change in the thinking of what is possible.

Single-cell analysis often focus on variation across clusters.
◼️ Yet, variation within clusters (conditions) is informative.

Proteins that do not change across conditions covary within a condition across single cells.

nature.com/articles/s4159… via Nature Methods
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When is single-cell analysis needed ?

Should you measure nucleic acids, proteins or metabolites?

How do you achieve robust results ?

mcponline.org/article/S1535-…

The regulatory capacity of dynamic systems is rich & fascinating.

They can enable a common regulatory hub to differentially regulate diverse physiological processes.

pubs.acs.org/doi/full/10.10…

#SCP2024 will be broadcast for remote attendance.

Do you remember the big questions that inspired you to become a scientist ?

Keep raising them.
◼️ Technological progress can convert scientifically intractable questions into tractable ones; they can be rigorously framed and empirically answered.

nikolai.slavovlab.net/Preserve-inspi…

Prof. Nikolai Slavov Slavov Laboratory Christian Montes Trevor Nolan Open, comprehensive, and detailed protocols were key. Thank you!

5 days until the deadlines for abstracts & scholarships for Single-Cell Proteomics Conference.

single-cell.net/proteomics/scp…

I still remember vividly the talks by Steve McCarroll and Ralph Weissleder.

Ralph presented a sweeping panorama of problems needing single-cell proteomics.

youtu.be/a_xt37XoLDg?si…

We first met in June 2018.
Since then, every meeting has reflected the annual progress of our community.

Based on the abstracts, 2024 will not disappoint.

I can barely wait for my teams to share results from Slavov Laboratory and Parallel Squared Technology Institute (PTI).

single-cell.net

Nanopore direct RNA seq & siRNA knockdowns help identify pseudouridine sites and their synthase enzymes.

Well done Sara Rouhanifard and team!

We are hiring !
The opportunities are for colleagues who want to pursue big bold ideas in a well-resourced non-profit environment.

We support open science & career growth.

Requirements: Enjoying challenges & collaborations.

parallelsq.org/careers

Please RT.

We are yet to investigate proteomes with unbiased discovery-driven methods.

Yet, the prospects are good.
We may soon enter an age of discovery when proteomes are routinely explored at scale and their secrets revealed.

nikolai.slavovlab.net/Proteome-secre…

Let's elevate our scientific discourse:

◼️ Replace adjectives with factual statements
◼️ Offer constructive criticism
◼️ Place results in context where they can be better appreciated
◼️ Make research results accessible to broader audiences

blog.slavovlab.net/2020/07/15/pro…

As we celebrated our 1st anniversary, we reflected on a year of building up, launching projects and establishing collaborations.

parallelsq.org/blog/celebrati…

I am frequently asked questions that are answered in this Nature Methods article.

So, I will re-share it.

nature.com/articles/s4159…

Shout out to Prof. Nikolai Slavov for a fantastic talk on high throughput #singlecell #proteomics by mass spec & 🙌🏻 for all their efforts on making this technique & downstream analysis accessible to the scientific community NextGen Omics

more info at slavovlab.net

Good point.
How should we name new phenomena that we do not yet understand ?

I favor phenomenological terminology describing the measurements; that's what we have used.

E.g., introducing high oxygen consumption (HOC) helped.

molbiolcell.org/doi/full/10.10…
pnas.org/doi/full/10.10…
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Proteins are dynamic.
These dynamics animate life.